Surprisingly, specific ETV6 probe (YAC 936E2) demonstrated two distinct locations of ETV6 amplification: in hsr for the clone with the der(12) of variable length and in small markers (mar2) for the second clone, each one being mutually exclusive. MFISH results confirmed the global structural abnormalities and did not show additional abnormalities. We next tried to demonstrate the specific amplification of classic tall uggs cheap ETV6 and quantify the mean number of ETV6 copies in each bone marrow cell. As no frozen cells were available, thus preventing Southern blot analysis, realtime quantitative DNA PCR was used in order to compare the copy number of ETV6 gene to the reference albumin (ALB) gene, located michael kors ladies watch on chromosome 4q11, in which no cytogenetic abnormality was detected.
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